Mesalmine Targeted Water-Compatible Molecularly Imprinted Polymer-Silver Nanoparticles with Surface-Enhanced Raman Spectroscopic (SERS) and Voltammetric Detection

摘要

Here, a facile and efficient approach to fabricate water-compatible molecularly imprinted nanoparticles selective for mesalmine has been attempted by using water soluble functional monomers for preparing the imprinted polymer network. On polymerization with mesalamine as template analyte, the adduct was attached to silver nanoparticles generated in situ. Surface enhanced raman spectra (SERS) was used to monitor the analyte uptake and removal with electrochemical detection as quantification technique. SERS offers a highly selective and sensitive probe for such template molecules, but the selective layer needs to be combined with a SERS-active metal surface. Cyclic voltammetry (CV) at glassy carbon electrode was used for quantification of mesalmine with Ag/AgCl as reference electrode and Pt wire as counter electrode. The effect of nanosize is manifested as the adsorption capacity is enhanced ～10 times. Detection limit for mesalmine template in aqueous solution by silver nanoparticles (AgNPs)-molecularly imprinted polymer (MIP) is 10.5 μg mL~(-1). Thus the enzyme-like selectivity and specificity of molecular imprinting technology can be enhanced manifolds by fabricating nanosized MIPs.