Interaction of unmodified and partially silylated nanosilica with red blood cells

摘要

Interaction of red blood cells(RBCs)with unmodified and partially(50%)silylated fumed silica A-300(nanosilica)was studied by microscopic,XRD and thermally stimulated depolarisation current(TSDC)methods.Nanosilica at a low concentration C_(A-300)<0.01 wt.%in buffered aqueous suspension is characterised by a weak haemolytic effect on RBCs.However,at CA?300=1 wt%all RBCs transform into shadow corpuscles because of 100%haemolysis.Partial(one-half)hydrophobization of nanosilica leads to reduction of the haemolytic e?ect in comparison with unmodified silica at the same concentrations.A certain portion of the TSDC spectra of the bu?ered suspensions with RBC/A-300 is independent of the amounts of silica.However,significant portions of the low-and high-temperature TSDC bands have a lower intensity at C_(A-300)=1 wt%than that for RBCs alone or RBC/A-300 at C_(A-300)=0.01 wt.%because of structural changes in RBCs.Results of microscopic and XRD investigations and calculations using the TSDC-and NMR-cryoporometry suggest that the intracellular structures in RBCs(both organic and aqueous components)depend on nanosilica concentration in the suspension.