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Specific expression of an expansin gene during elongation of cotton fibres

机译:棉纤维伸长过程中expansin基因的特异性表达

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A differential screening experiment resulted in the isolation of a full-length cDNA clone encoding an expansin from cotton fibres. Nucleotide and derived amino acid sequence data showed that pGhEX1 encodes an expansin of 258 amino acids, with an N-terminal signal peptide. Northern blot analysis showed that the corresponding transcript is abundant in cotton fibre cells but absent in all other tissues tested, and that the gene is developmentally regulated during fibre elongation.
机译:差异筛选实验导致从棉纤维中分离出编码扩展酶的全长cDNA克隆。核苷酸和衍生的氨基酸序列数据表明,pGhEX1编码一个258个氨基酸的弹性蛋白,带有一个N端信号肽。 Northern印迹分析表明,在棉纤维细胞中相应的转录本丰富,但在所有其他测试组织中却不存在,并且该基因在纤维伸长过程中受到发育调控。

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