Chromosomal localization of silkworm (Bombyx mori) sericin gene 1 and chymotrypsin inhibitor 13 using fluorescence in situ hybridization

摘要

The chromosomal locations of two single-copy genes,Ser-1 and CI-13,in silkworm (Bombyx mori) were detected at the molecular cytogenetics level by fluorescence in situ hybridization in the study.The results showed that Ser-1 is located near the distal end of the 11th linkage group,relatively at the 12.5±1.4 position in pachytene;and that CI-13 has been mapped near the distal end of the 2nd linkage group,relatively at the 8.2+-1.2 position in pachytene.Furthermore,their location model map-FISH map on silkworm chromosome was drawn.The FISH technique and its application to silkworm are also discussed in this paper.