Somatic Embryogensis using Cucumis sativus L. cotyledons

摘要

General problems with in vitro culture of cucumber (Cucumis sativus L.) include abnormal embryo development, poor differentiation of callus into shoots, poor survival of regenerated plants in soil, and undesirable changes in ploidy level of regenerated plants (Punja et al., 1990; Chee, 1990). Cucumber somatic embryos have been derived using different explant sources, cultivars, growth regulator combinations, gelling agents and carbohydrates (Kim et al., 1988; Kim and Janick, 1989; Cade et al., 1990; Chee, 1990; Punja et al., 1990; Ladyman and Girard, 1992; Lou and Kako, 1994), and somatic embryos have been induced from various explants, such as cotyledons, leaves, hypocotyls and stem internodes. A high frequency of abnormal embryogenesis, such as disordered bipolarity; embryo death, arrested growth, re-callus, and vitrification of embryos has been reported for cucumber (Chee, 1990; Ladyman and Girard, 1992; Lou and Kako, 1994). With these problems, the average frequencies of regeneration of whole cucumber plants with normal morphogenesis have been small.