The genetic linkage maps of Anthurium species based on RAPID, ISSR and SRAP markers

摘要

We report the construction of genetic linkage maps of two species Anthurium ornatum (A. ornatum) and Anthurium andreanum (A. andreanum) based on random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR) and sequence related amplified polymorphism (SRAP) markers using a double-pseudo test cross mapping strategy. Mapping population of 43 F1 individuals was developed using a natural mutant IIHR selection A1 (A. ornatum or Anthurium nymphaefolium) and a variety called Eternity (A. andreanum). A total of 228 markers, including 99 RAPDs, 21 ISSRs and 108 SRAP combinations were used for the linkage group analysis. The linkage map of A. ornatum consisted of 10 linkage groups and a doublet covering 1233.5 cM, with a total of 85 markers, whereas the linkage map of A. andreanum consisted of 12 linkage groups covering a distance of 1023.5 cM with 78 markers. The constructed linkage map covered a total genome length of 77% and 73% of A. ornatum and A. Andreanum, respectively. This study provides an important basis for identification and mapping of horticultural traits, which would be exploited in Marker-Assisted Selection in Anthurium breeding programs