Distribution of Bone-Marrow Stromal Cells in a 3D Scaffold Depending on the Seeding Method and the Scaffold Inside a Surface Modification

摘要

The distribution of bone-marrow stromal cells (BMSC) was studied in 3D polylactide scaffolds. Seeding of cells into the scaffold by the dynamic method (with the aid of a peristaltic pump) has been shown to provide distribution of cells throughout theentire scaffold volume, unlike the static method of seeding, in which the cell suspension is applied onto the scaffold surface. Unlike the cells seeded into the scaffold by the dynamic method, the cells seeded by the static method practically completelymigrate from the scaffold on the dish for the first several days. It is revealed that BMSCs cultivated in 3D polylactide scaffolds modified by fibrin form colonies, whereas BMSCs cultivated inside scaffolds modified by collagen type 1 distribute all overthe scaffold volume in the form of individual cells.