In vitro bonelike apatite formation on magnetite nanoparticles after a calcium silicate treatment: Preparation, characterization and hemolysis studies

摘要

Bioactive magnetite nanoparticles were prepared successfully by coating magnetite nanoparticles with CaSiO_3 followed by their immersion in simulated body fluid. The Fe_3O_4 nanoparticles (5-10 nm) were synthesized by a co-precipitation technique. In order to prepare core-shell nanocomposites, the nanoparticles were soaked for 1 h in a calcium silicate solution that had been aged for 24 h before using it. The samples were dried in air and then immersed in SBF at 37 °C for 1, 3 and 7 days. The analyses of the samples after the biomimetic process revealed the formation of a bonelike apatite layer on all the samples tested and not a significant change was observed on their original magnetic behavior. Hemolysis test, evaluated as release of hemoglobin, revealed that all the samples showed no hemolytic effects up to 3 mg/ml, indicating no damage of the red blood cell membranes. These bioactive, hemocompatible and superparamagnetic particles may be potential materials for bone cancer treatment by hyperthermia.