L'IGF-1 induit une augmentation de la taille et du contenu en myosine des myotubes humains IGF-1 induces an increase in the size and in the myosin content of human myotubes

摘要

Introduction.-In this study, we tested the ability of IGF-1 (insulin-like growth factor-1) to increase the size of human myotubes in culture.Synthese des faits.-IGF-1 treatment induces hypertrophy of human myotubes characterized by a dose dependant increase in the mean number of nuclei per myotube, in the size of the myotubes and a significant increase of 96,4 (+-)50,48% (P=0,002) of the ratio myosin/emerine.Conclusion.- In this study, we showed that IGF-1 induces an increase in the size of human myotubes in culture and increases myosin synthesis, thus mimicking the hypertrophy described in vivo. This model will be used for further analysis of the molecular mechanisms involved in this hypertrophy.