Interaction between Non-Histone Chromatin Protein HMGB1 and Linker Histone H1

摘要

The fundamental possibility of interactions between non-histone chromatin protein HMGB1 and linker histone H1 in solutions with different ionic strengths was studied by intrinsic UVfluorescence, far and near UV CD, and spectrophotometry. The data we obtained allow us to assume that the increase in the histone H1 content in HMGB1 solutions with low ionic strengths is accompanied by the destruction of HMGB1 associates. The interactions between HMGB1 and H1 proteins increase the number of ordered regions in the protein molecules and causes slight changes in the tertiary structure of the protein.