Replication of H9N2 avian influenza virus in lungs and kidneys after inoculation in broilers.

摘要

H9N2 avian influenza subtype occurs in many Eurasian countries producing high mortality in chicken farms. RT-PCR assay was performed to compare the duration of H9N2 avian influenza viral replication in the lung and kidney of broiler chicken after inoculation. Thirty day old commercial broilers were inoculated intranasaly with H9N2 low pathogenic avian influenza virus. Five of these had lung and kidney tissues taken at 3, 7, 11 and 15 post inoculation for viral detection by TR-PCR. Virus was detectedat in lungs at day 3(20% of chickens), 7(40%), 11(20%) and 15(0%) after inoculation. Virus detected in kidneys at 3(%20 chickens) ,7(%80) ,11(%20) ,15(%20) days post inoculation. The results suggest that H9N2 avian influenza subtype can be replicated and isolated from kidneys for a longer time.