Human mesenchymal stem cell viability, proliferation and differentiation potential in response to ceramic chemistry and surface roughness

摘要

We investigated the effect of the ceramic chemistry and surface roughness of pure alpha-tricalcium phosphate, and also alpha TCP doped with either 1.5 wt% or 3.0 wt% of dicalcium silicate (C2S), on the response of adult human mesenchymal stem cells (ahMSCs). AhMSCs were plated onto ceramic discs, prepared by a solid-state reaction. After being sintered, some samples were polished up to 1 mu m, while others were kept as manufactured, which resulted in two surface roughness grades. Viability, proliferation and osteoinductive capacity were determined following various incubation periods.