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Drought tolerance in recombinant inbred lines (RILS) derived from the cross of BPT-5204/Sahbhagi dhan

机译:BPT-5204 / Sahbhagi dhan杂交衍生的重组自交系(RILS)的耐旱性

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Drought stress is a major constraint for rice (Oryza sati va L.) production and yield stability in rainfed ecosystems. Higher probability and greater spatial covariance of drought and less diversified farming systems with rice accounting for a larger share of household income are likely to be the main reasons for this higher cost of drought in eastern India. Farmers deploy various coping mechanisms but such mechanisms are largely unable to prevent a reduction in income and consumption, especially in eastern India. Therefore, identifying drought tolerant genotypes will help to develop rice cultivars suitable for water-limiting environments through marker-assisted breeding. F' progenies derived from the cross of BPT 5204 and Sahbhagi dhan were used to generate the RILs (Recombinant Inbred Lines) by advancing the F' generation to Fg Eight hundred ten RILs were screened for drought tolerance in the rainout shelter. Scoring for leaf rolling, leaf drying and recovery was based on the 0-9 scale ofSES (Standard Evaluation System) oflRRI. The screening results showed one hundred forty recombinant inbred lines were found highly resistant to drought (scale 0-3). Further confirmation of drought tolerance was based on the use of polymorphic microsatellite markersRM 3825, RM 1349, RM 24, RM 232, RM 212 and RM 3 linked to different drought related characteristics such as plant height, panicle length, test weight and grain yield.
机译:干旱胁迫是雨养生态系统中水稻(Oryza sati va L.)生产和产量稳定的主要限制因素。干旱的较高概率和较大的空间协方差,以及耕作制度多样化程度较低,其中水稻占家庭收入的比重较高,这很可能是印度东部干旱成本上升的主要原因。农民采用了各种应对机制,但这种机制在很大程度上无法防止收入和消费的减少,特别是在印度东部。因此,鉴定耐旱基因型将有助于通过标记辅助育种发展出适合水限制环境的水稻品种。通过将F'代提高到Fg,使用了BPT 5204和Sahbhagi dhan杂交得到的F'后代来生成RIL(重组自交系)。在雨棚中筛选了810个RIL,以耐干旱。叶片卷叶,叶片干燥和恢复的评分基于1RRI的SES(标准评估系统)的0-9评分。筛选结果表明,一百四十个重组自交系被发现具有高度抗旱性(0-3级)。抗旱性的进一步确认是基于多态性微卫星标记RM 3825,RM 1349,RM 24,RM 232,RM 212和RM 3的使用,这些标记与不同的干旱相关特性(例如株高,穗长,试验重量和籽粒产量)相关。

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