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Estimation of genetic diversity among parents and F3 mapping population developed between a salt tolerant and salt susceptible rice variety

机译:在耐盐和耐盐水稻品种之间发展了亲本和F3作图群体之间的遗传多样性估计

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摘要

Hybridization was made between a high yielding, salt susceptible rice variety IR 28 and a salt tolerant rice cultivar Pokkali to generate a mapping population aiming to tag the salt tolerant gene(s) in rice. Phenotyping of 129 F} segregants was done which showed varying degree of tolerance towards excess salt (12 dSm~' NaCl) provided artificially. Phenotyping of the segregants and parents was done based upon tolerance (0-9 scale) towards salt stress. Three groups (highly tolerant, 0-1, moderately tolerant, 2-4 and susceptible 5-9) were formed based upon tolerance norms. Genetic analysis using RAPD grouped the segregants cultivar into two major classes with two sub-classes each. RAPD was found to be potential to tag the gene(s) as indicated by character analysis using UPGMA
机译:在高产,耐盐水稻品种IR 28和耐盐水稻品种Pokkali之间进行杂交,以产生一个定位种群,旨在标记水稻中的耐盐基因。对129 F}分离剂进行了表型鉴定,显示出对人工提供的过量盐分(12 dSm〜NaCl)的耐受程度不同。基于对盐胁迫的耐受性(0-9标度)进行分离物和亲本的表型鉴定。根据耐受标准形成三组(高耐受性,0-1,中耐受性,2-4和易感性5-9)。使用RAPD进行的遗传分析将分离子品种分为两个主要类别,每个子类别两个。如使用UPGMA进行的字符分析所示,发现RAPD有潜力标记基因

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