Synthesis of a 2'-Se-uridine Phosphoramidite and Its Incorporation into Oligonucleotides for Structural Study

摘要

We report here the synthesis of the 5'-[benzhydryloxybis(trimethylsilyloxy)]silyl-2'-methylseleno-2'-deoxyuridine phosphoramidite and its incorporation into Oligonucleotides by solid-phase synthesis.The coupling yield of this phosphoramidite into Oligonucleotides is higher than 99%.We also demonstrate that this 2'-methylselenophosphoramidite is compatible with the 5'-silyl-2'-ACE chemistry,for longer Se-RNA solid-phase synthesis.Our preliminary NMR study on the synthesized 2'-Se-DNA has revealed a U_(Se)-A base pair and a duplex structure formation when its complementary strand was present.