New sources of cytoplasmic genie male sterility in rice

摘要

In rice (Oryza sativa), wild species are important sources of cytoplasmic genie male sterility (CMS). Fifty six inter specific hybrids were made and evaluated for pollen and spikelet fertility. The male sterility source was identified by reciprocal difference. The three hybrids viz., O.nivara 1053431 CO 45, O.barthii 1009341IR 50, O.nivara 1015081 IR 64 were found sterile and substitution backcrosses were made with their respective male parents to develop new CMS lines. The hybrid O.nivara 1058791 CO45 with high reciprocal difference was selfed and F_2 plants were observed for sterility. One plant was identified as sterile in a population of 74 plants and backcross was also made using the respective male parent. Preliminary studies on characterization of four new CMS lines through fertility restoration revealed that these sources were different from existing WA source and also from one another.