Two New Chiral EDTA-Based Metal Chelates for Weak Alignment of Proteins in Solution

摘要

A short synthesis of EDTA-based metal chelates that can be attached to the cysteine residue of a protein via a disulfide bond is described.The complexes were used after coordination of lanthanides to align trigger factor and apo-calmodulin in solution to yield residual dipolar couplings and pseudocontact shifts.Alignment tensors for the new tags are linearly independent compared to those of previously published tags.