Study of 1-Deoxy-D-xylulose-5-phosphate Reductoisomerase:Synthesis and Evaluation of Fluorinated Substrate Analogues

摘要

1-Deoxy-D-xylulose-5-phosphate(DXP)reductoisomerase is a NADPH-dependent enzyme catalyzing the conversion of DXP to methyl-o-erythritol 4-phosphate(MEP).In this study,each of the hydroxyl groups in DXP and one of its C-1 hydrogen atoms,were separately replaced with a fluorine atom and the effect of the substitution on the catalytic turnover was examined.It was found that the 1-fluoro-DXP is a poor substrate,while both 3-and 4-fluoro-DXP behave as noncompetitive inhibitors.