Development of two powdery mildew and stripe rust resistant wheat lines from (Triticum turgidum x Haynaldia villosa amphiploid) x synthetic wheat hybrids.

摘要

Among the progenies of crossing Triticum turgidum - Haynaldia villosa amphiploid with synthetic hexaploid wheat (T. carthlicum/Aegilops tauschii) Am3, two lines (SN030713 and SN05078), with good resistance to stripe rust and powdery mildew, were developed. Cytological studies demonstrated that SN030713 contained 42 chromosomes and formed 21 bivalents at meiotic metaphase I. SN05078 contained 28 chromosomes and formed 14 bivalents. Genomic in situ hybridization analysis using H. villosa V genomic DNA as the probe showed SN030713 and SN05078 had no large H. villosa chromosome fragments. PCR analysis with H. villosa specific primer pHv29 showed that H. villosa genetic materials were introgressed in these two lines. SSR analysis indicated that the genomic composition of SN030713 was 2n=6x=42 (AABBDD), and SN05078 was 2n=4x=28 (AABB). Introgressed Ae. tauschii genetic materials in SN05078 were also detected.