THE EFFECT OF PARAQUAT ON FLAVONOID PRODUCTION IN FAGOPYRUM ESCULENTUM CULTURES IN VITRO

摘要

Oxidative stress is a major damaging mediator in plants exposed to a variety of stresses such as drought, low temperature, air polutants and chemicals including paraquat. Paraquat/methylviologen/ (MV) is widely used as nonselective contact herbicide and as crop dessicant on products. Pesticides may induce oxidative stress, leading to generation of free radicals and alternative in antioxidants, oxygen free radicals, the scavenging enzyme system and lipid peroxidation. Buckwheat is a valuable source of flavonoid of rutin. The effect of 6, 12, 24, 48, 72 and 168 hours treatment of MV in 3 various concentrations on rutin production by Fagopyrum esculentum callus and suspension cultures was tested in this study. The maximum rutin content (0.25 %) in callus culture was demonstrated after 72 hours of MV elicitation in concentration of 2.1929.10(3) mol/L. The maximum rutin content in suspension culture (0.02 %) was reached after 24 hour of MV treatment in concentration of 2.1929.10-5 mol/L.