Polyclonal antibodies to grain gliadins and their application in wheat quality prediction.

摘要

Gliadins were extracted from 3 wheat varieties, Nongda 99260037 (good quality), Henan 9023 (medium quality) and Nongda 98123 (poor quality), and alpha-, beta-, gamma-, omega -gliadins were purified from Nongda 99260037 using preparative SDS-PAGE. The total gliadins and a-, beta-, gamma-, omega -gliadins were used as antigens to immunise BALB/C mice and the corresponding polyclonal antibodies were prepared, designated as Anti-A, Anti-B, Anti-C, Anti-A alpha , Anti-A beta , Anti-A gamma and Anti-A omega, respectively. Binding of the polyclonal antibodies with 8 wheat varieties, that varied in quality properties, showed correlations with some wheat quality parameters. Correlation coeff. between antibodies against total gliadins or gamma -, and omega -gliadin of Nongda 99260037 and quality parameters were higher than other antibodies. Of 7 polyclonal antibodies tested, 3 (Anti-A gamma, Anti-A omega and Anti-A) displayed significant positive or negative associations between antibody binding and dough development time, strength, valorimenter value and stability time but no significant correlations were observed with water absorption. These results suggest that polyclonal antibodies could be used for rapid prediction and screening of wheat quality parameters.