Measurement of melamine migration from melamine-ware products by designed HPLC method and the effect of food-type on the level of migration

摘要

© 2018 Interdisciplinary Toxicology, published by Sciendo 2018.Melamine-ware is widely used around the world. There is a public health concern as regards the safety of melamine when exposed to food. This study was carried out to measure the level of melamine migration in melamine-ware products by HPLC method and the effect of food-type on the level of melamine migration. In food control laboratories in Iran, there is no common method to measure and monitor melamine migration, hence a method using HPLC technique was adopted and validated to solve this problem. The validation results showed the reliability with 94.9 accuracy and 95.3 precision. Furthermore, the limit of detection (LOD) and quantification (LOQ) were 0.145 and 0.435 μg/ml, which for a new method were within acceptable ranges. Melamine migrations from 4 most available melamine wares were measured. Distilled water, 3 acetic acid and 15 ethanol were used as food simulant at 30 °C for 90 min. Although melamine migration occurred in all samples and acidic conditions had a significant effect, the values were not higher than the European standard (30 μg/ml). The study revealed that the HPLC method was valid and could be applied and developed to measure melamine migration. However, precautions should be considered while choosing melamine-ware utensil as long-term exposure to this substance has a negative effect on health, especially on the kidneys.