Kinetic and Mechanism of Reactions of L-α-Glutamic Acid and L-Glutamine with Pyridoxal

摘要

The kinetics and mechanisms of condensation of pyridoxal with L-α-glutamic acid and L-glutamine were studied by UV spectroscopy and polarimetry. L-a-Glutamic acid reacts with pyridoxal to form a Schiff base whose subsequent hydrolysis gives rise to pyridoxamine and a-ketoglutaric acid. The reaction of L-glutamine with pyridoxal involves the γ-NH2 group and affords a Schiff base whose subsequent hydrolysis gives rise to pyridoxamine and L-α-glutamic acid.