Torularhodin Biosynthesis and Extraction by Yeast Cells of Rhodotorula Rubra

摘要

The paper presents the research work done to study the discontinuous bioprocess for the intracellular carotenoidic pigment - torularhodin formation with the yeast Rhodotorula rubra ICCF209. The growth and carotenoid biosynthesis of the yeast Rhodotorula rubra was studied by cultivation in 3.7 L Bioengineering AG tab bioreactor with mechanical stirring at an air flow rate of 200 L/h and agitation at 600 rpm for 4 days. The medium composition, defined as MS3, was obtained with the formula: 30 g/L glucose, 1.5 g/L yeast extract, 5 g/L NH4NO3, 1 g/L KH2PO4, 0.4 g/L MgSO4 x 7H2O, 0.4 g/L NaCl and 1 g/L alanine. The bioreactor was equipped with computerized control units for temperature, air flow, stirrer speed, pH, and pO2, and acquisition data every 15 s was programmed.