Construction of normal human IgE phage antibody library and the screening of the anti-trichosanthin IgE

摘要

Allergen specific IgE response is the major cause of immediate hypersensitivity. However the number of IgE-producing B cells and the amount of IgE, especially the specific IgE, are so low, it greatly impedes the study of the allergic-specifc antibodyresponses. Here we report the construction of a normal human IgE combinatorial library. The repertoire of IgE VH genes and of K genes were separately amplified from normal human peripheral blood lymphocytes through RT-PCR, and were then constructed to form the phage surface display human Fab(IgEVH) library. A plant protein allergen, trichosanthin(TCS), was used to affinity-enrich and to screen the anti-TCS phage HuFab clones from the library. Human IgE(Fab) to TCS were detected.