Multivariate analysis of biological nitrogen fixation and seed yield in Iranian common bean (Phaseolus vulgaris L.) genotypes.

摘要

In order to improve the biological nitrogen fixation and seed yield in Iranian common bean germplasm, 64 genotypes collected from different origins were cultivated in two separate randomized complete block designs. The genotypes were inoculated by bacteria Rhizobium legominosarum biovar. Phaseoli isolate L-109 only in one of the experiments. Second experiment was considered as check for the first. Standard deviation and analysis of variance revealed genetic diversity among common bean genotypes for all the traits. Factor analysis based on principal component analysis method and varimax rotation indicated that three important factors accounted for about 94.15% of the total variation among traits. The first factor assigned 52.49% of total variation between traits and was significantly related with biological nitrogen fixation, nodule number/plant, total nitrogen of shoot, harvest index, 100-seed weight and pod number/plant. This factor was titled as nitrogen fixation ability factor. Therefore, the traits related with this factor can be used for genetic improvement of nitrogen fixation ability in common bean genotypes. The second factor accounted for 31.72% of total variation and was correlated with traits seed yield, pod yield/plant and biological yield. This factor was considered as seed production factor and was proposed for increasing seed production in this crop. Result of factor analysis was confirmed using cluster analysis of variables.