首页> 外文期刊>Biochimica et biophysica acta. Gene structure and expression >Transfection of TTF-1 gene induces thyroglobulin gene expression in undifferentiated FRT cells
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Transfection of TTF-1 gene induces thyroglobulin gene expression in undifferentiated FRT cells

机译:TTF-1基因的转染诱导未分化的FRT细胞中甲状腺球蛋白基因表达

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摘要

The thyroglobulin gene, the substrate for thyroid hormone biosynthesis, is not expressed in the FRT cell line, which, even though it manifests the polarised epithelial phenotype, does not express any of the thyroid functional properties. Two transcription factors, TTF-1 and Pax-8, have been implicated in thyroid specific expression of the thyroglobulin gene. FRT cells contain Pax-8 but they lack TTF-1. In this paper, we show that transfection of TTF-1 expression vectors in FRT cells results in activation of thyroglobulin gene expression. If the expression vector encoded for TTF-1–ER, a fusion gene coding for the entire TTF-1 protein fused to the hormone-binding domain of the steroid receptor, under the control of the RSV promoter, thyroglobulin gene expression was controlled by estrogen. These data provide a direct demonstration that TTF-1 activates the chromosomal thyroglobulin promoter. Since transfection of TTF-1 expression vectors in non-thyroid cell types did not result in thyroglobulin gene expression, it is suggested that Pax-8, in addition, perhaps, to a specific cellular environment, might be required for thyroid specific expression of the thyroglobulin gene.
机译:甲状腺球蛋白基因是甲状腺激素生物合成的底物,在FRT细胞系中不表达,即使它表现出极化的上皮表型,也不表达任何甲状腺功能特性。甲状腺球蛋白基因的甲状腺特异性表达涉及两个转录因子TTF-1和Pax-8。 FRT细胞含有Pax-8,但缺少TTF-1。在本文中,我们显示TTF-1表达载体在FRT细胞中的转染可导致甲状腺球蛋白基因表达的激活。如果表达载体编码为TTF-1–ER,这是一种编码完整TTF-1蛋白的融合基因,该蛋白与类固醇受体的激素结合域融合,在RSV启动子的控制下,甲状腺球蛋白基因的表达受雌激素控制。 。这些数据直接证明了TTF-1激活了染色体甲状腺球蛋白启动子。由于TTF-1表达载体在非甲状腺细胞类型中的转染不会导致甲状腺球蛋白基因表达,因此建议除了特定的细胞环境外,可能还需要Pax-8来实现甲状腺的特定表达。甲状腺球蛋白基因。

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