Identification of polymorphisms and association analysis with meat quality traits in the porcine KIAA1717 and HUMMLC2B genes

摘要

Skeletal muscle genes are potential candidates for production and meat quality. Screening a subtracted cDNA library constructed with mRNA obtained from longissimus dor si muscles of Fl hybrids Landrace x Yorkshire and their female parents Yorkshire, we isolated two partial sequences coding for the H3-K4-specific methyltransferase (KIAA1717) and skeletal muscle myosin regulatory light chain (HUMMLC2B) genes. Database search revealed KIAA1717 and HUMMLC2B encoded proteins with SET domain and EF-hand calcium binding motif, respectively. In the present work we identified their partial polymorphisms and two SNPs, one (C1354T) at the 3' untranslated region (UTR) of KIAA1717 and one (A345G) at the SINE (PRE-1) element of HUMMLC2B, both createdidisrupted arestriction site for endonuclease Msp I. The selected pigs were genotyped at the KIAA1717 C1354TandirtiMMLC25 A345G sites by means of a PCR-RFLP protocol. Significant associations were observed for the KIAA1717 C1354T polymorphic site with meat marbling(longissimus doris (p<0.05), biceps femoris (p<0.01)) and intramuscular fat (p<0.01). HUMMLC2B A345G were significantly associated with meat pH (longissimus doris (p<0.05), biceps femoris (p<0.01)), drip loss (p<0.01), water holding capacity (p<0.01) andmeat color value (longissimus doris (p<0.01), biceps femoris (p<0.05)). Further studies are needed to confirm these preliminary results.