Enzymatic degumming of soybean oil with magnetic immobilized phospholipase A(2)

摘要

In this study, magnetic immobilized phospholipase A(2) (PLA(2)) was used to degum soybean oil such as magnetic particles crosslinked with sodium alginate PLA(2) (PLA(2)-MPCSA), Magnetic chitosan micropartides containing PLA(2) (PLA(2)-MCM), Fe3O4/P(GMA-EDGM-St) PLA(2), and Fe3O4/SiOx-g-P(GMA) PLA(2), successfully improving the recycling aspect of the degumming process. The highest enzyme loading and enzymatic activity (122.60 mg/g and 1289 U/g, respectively) were obtained using the magnetic immobilized PLA(2)-Fe3O4/SiOx-g-P(GMA) system. Compared to free PLA(2), magnetic immobilized PLA(2) had a broader pH-activity profile (pH 4.0-5.5) and activity remained stable between 50 and 70 degrees C. Enzymatic degumming with PLA(2)-Fe3O4/SiOx-g-P(GMA) was carried out over 5.0 h with an enzyme dosage of 0.24 g/kg, a pH of 5.0, and a temperature of 55 degrees C, resulting in residual phosphorus and free fatty acid contents of 9.8 mg/kg and 0.84 g/100 g, respectively. Moreover, magnetic immobilized PLA(2) retained more than 80% of its initial activity even after 5 cycles of soybean oil degumming. (C) 2016 Elsevier Ltd. All rights reserved.