Novel folate binding protein-1 interactions in embryonic orofacial tissue

摘要

Aim: To identify proteins with which FolBpl may interact within lipid rafts in tissue derived from embryonic orofacial tissue.Methods: A yeast two-hybrid screen of a cDNA library, derived from orofacial tissue from gestational day 11to 13 mouse embryos, was conducted.Key findings: Using the full-length FolBpl protein as bait, two proteins that bind FolBpl were identified,Bat2d, and a fibronectin type Ill-containing domain protein. Results were confirmed by glutathione S-transferase pull-down assays.Significance: As a component of membrane lipid raft protein complexes, these binding proteins may represent "helper" or chaperone proteins that associate with FolBpl in order to facilitate the transport of folate across the plasma membrane. The protein-protein interactions detected, while limited in number, may be critical in mediating the role of FolBpl in folate transport, particularly in the developing embryo.