Expression profile of Notch-1 in mechanically overloaded plantaris muscle of mice.

摘要

AIM: We investigated the expression pattern of Notch-1 in normal and hypertrophied plantaris muscle of mice. MAIN METHODS: We performed immunofluorescence of both Notch-1 and the Notch-1-linking molecules. KEY FINDINGS: Immunofluorescence labeling revealed Notch-1 protein in Pax7-positive satellite cells during days 2-6. We observed clear co-localization between Notch-1 and myogenin (4.9+/-1.3%) in the hypertrophied muscle at 4days. Several mononuclei (possibly satellite cells) possessed both Notch-1 and Foxo1 in the plantaris muscle subjected to mechanical overloading (4.1+/-1.2%). SIGNIFICANCE: Notch-1 may play an important role in the maintenance of quiescent satellite cells.