Highly sensitive electrochemiluminescence determination of etamsylate using a low-cost electrochemical flow-through cell based on a tris (2, 2’-bipyridyl)ruthenium(II)–Nafion-modified carbon paste electrode

摘要

A simple and sensitive electrochemiluminescence (ECL) method for the determination of etamsylate has been developed by coupling an electrochemical flow-through cell with a tris(2,2’-bipyridyl)ruthenium(II) (Ru(bpy)_3~(2+))–Nafion-modified carbon electrode. It is based on the oxidized Ru(bpy)_3~(2+) on the electrode surface reacting with etamsylate and producing an excellent ECL signal. Under optimized experimental conditions, the proposed method allows the measurement of etamsylate over the range of 8–1000ng/mL with a correlation coefficient of r=0.9997 (n = 7) and a limit of detection of 1.57 ng/mL (3σ), the relative standard deviation (RSD) for 1000 ng/mL etamsylate (n=7) is 0.96%. The immobilized Ru(bpy)_3~(2+) carbon paste electrode shows good electrochemical and photochemical stability. This method is rapid, simple, sensitive and has good reproducibility. It has been successfully applied to the determination of the studied etamsylate in pharmaceutical preparations with satisfactory results. The possible ECL reaction mechanismhas also been discussed.