The development of an indirect ELISA for the detection of antibodies to goose parvovirus in blood serum.

摘要

This work aimed to develop an indirect enzyme-linked immunosorbent assay (i-ELISA), based on the structural protein VP2 of goose parvovirus (GPV), for the detection of antibodies to GPV in blood serum. In this study, the capsid protein VP2 of GPV was expressed in Escherichia coli and purified by nickel chromatography. The purified protein was capable of reacting with GPV antibodies, as analysed by Western blot. An i-ELISA based on the protein VP2 was developed. For evaluation of the newly developed ELISA, 192 field sera collected from six goose herds were tested in parallel by the serum neutralization (SN) test. The result showed that 138 samples were positive and 49 negative in both cases. The agreement of the two tests was 97.3%. This i-ELISA has high sensitivity and good specificity and is an alternative serologic test for the detection of the antibodies to GPV in blood serum.