Regeneration of Solanum tuberosum L. cv. Tomensa: induction of somatic embryogenesis in liquid culture for the production of 'artificial seed'

摘要

Somatic embryogenesis from excised shoot tip meristems of a tetraploid potato cultivar (Solanum tuberosum cv. Tomensa) was studied. Shoot tip meristems were excised from 3 - 4 weeks old in vitro propagated plants. Calli were induced in medium supplemented with 3 mg/l NAA and 0.25 mg/l BAP. After cultivation of the resulting calli in multiplication medium supplemented with 3 mg/l NAA and 1 mg/l BAP for 15 - 20 days, the hard green calli were transferred to embryo formation medium supplemented with 0.1 mg/l GA3 and 0.05 mg/l zeatin. Embryo formation was detected after 30 - 35 days. Histological studies confirmed the bipolar nature of the embryos. They developed to normal plants when transferred to soil and grown in a greenhouse.