Labeling of Oligopeptides with Luminescent Lanthanide(III) Chelates on Solid Phase

摘要

Synthesis of a building block based on 6,6'-(1H-pyrazole-1,3-diyl)bis(pyridine) (3) that allows introduction ofphotoluminescent lanthanide(III) chelates to the synthetic oligopeptides on solid phase using the standard Fmoc chemistryis described. Since the ligand based on (3) forms luminescent chelates with Eu~(3+), Sm~(Tb~(3+)and Dy~(ions, the ligand ishighly useful for multiparametric homogenous assays simplifying considerably the preparation of chelates needed in theseapplications.