Stimulated Raman scattering microscopy for live-cell imaging with high contrast and high sensitivity

摘要

We describe the principle and experiment of stimulated Raman scattering (SRS) microscopy, which has various advantages such as high-contrast and high sensitivity. To discuss how these advantages are realized in SRS microscopy, we introduce an intuitive picture of SRS, where the SRS process is viewed as homodyne detection of a nonlinear-optical signal by the excitation pulse.