Increased nitric oxide formation followed by increased arginase activity induces relative lack of arginine at the wound site and alters whole nutritional status in rats almost within the early healing period

摘要

Wound healing consists of a perfect and coordinated cascade of events that result in tissue reconstitution where local supplies with nutrients as well as general nutritional status have important roles [1]. Amino acid profiling of biological samples are good indicators of catabolic and nutritional status of an organism as well as a useful tool for understanding metabolic implications under various physiological conditions. [2-5]. It is known hydroxylation of phen-ylalanine (Phe) to tyrosine (Tyr) is considered to be the most important determinant of Phe catabolism occurring predominantly in the liver [6]. On the other hand plasma molar Phe to Tyr ratio is considered good indicator of net protein catabolism in the periph-eral tissue, particularly following injuries [7-9]. Branched-chain amino acids (BCA), comprising valine (Val), leucine (Leu) and isoleucine (Ile), are catabolized in peripheral tissues [10,11]. These amino acids are not only structural constituents of proteins but they are considered as regulators of insulin signaling [12]. Also, it has been shown that circulating BCA vs. aromatic amino acids (AAA) is a good indicator of liver energy status [13], while nutri-tional status of an organism is reflected on molar ratios between glycine (Gly) to BCA and alanine (Ala) to BCA in plasma [5].