Effects of putrescine on oxidative stress induced by hydrogen peroxide in Salvinia natans L.

摘要

Salvinia natans L. response to hydrogen peroxide (H 2 O 2 ) induced oxidative stress through physiological activities was evaluated. The plants were incubated with varying concentrations (0, 50, 100 µM) of H 2 O 2 and 100 µM of H 2 O 2 supplemented with 1 mM putrescine (Put) in hydroponic culture. This is observed with the decline in proline content and its biosynthetic enzymes viz. γ -glutamyl kinase and γ -glutamyl phosphate reductase activity. Protein carbamylated derivative by protein oxidation was another trait for oxidative damages by H 2 O 2 . The antioxidative enzymes like guaiacol peroxidase (GPX), glutathione reductase (GR), and catalase (CAT) recorded to express through in-gel staining with the H 2 O 2 exposure. On nuclear level, plants were sensitive to H 2 O 2 where the DNA disintegration was studied with comet assay and maximum comet tail observed at 100 µM H 2 O 2 treatment. Application of Put reduced the generation of protein oxidation and comet tail length as well as moderated the enzyme activity as revealed through in-gel staining.