Noninvasive detection of beta-carotene and lycopene in human skin using Raman spectroscopy

摘要

The antioxidant beta-carotene and lycopene substances were detected noninvasively, in vivo in human skin using Raman resonance spectroscopy. Both substances were detected simultaneously. To distinguish between the substances, the Raman signals were excited at 488 nm and 514.5 nm simultaneously using a multiline tiline Ar+ laser. The application of a fiber-based optical imaging system allowed the detection of P-carotene and lycopene on any skin area. The disturbance of the measurements because of nonhomogeneous skin pigmentation was avoided by using a measuring area of 28 mm 2 mm(2). The Raman spectroscopic method is well suited for the evaluation of the efficacy of topically or systemically applied amounts of beta-carotene and lycopene.