Photodynamic therapy for Photogem and Photofrin using different light wavelengths in 375 human melanoma cells

摘要

Melanoma is the most lethal of all cutaneous malignancies and additional treatment paradigms are needed. We explored the activity of PDT on human A-375 melanoma cells in vitro with different light wavelengths using porphyrin photosensitizers. The effect of red, green, and blue light in the cytotoxicity of these cells was measured, and the results suggest that there are no differences in the photodynamic activity of Photofrin and Photogem when irradiated with the blue, green and red light, if the effect of the molar absorption coefficient and the effect of light penetration in the tissue are taking into account. We report the ID{sub}10 (irradiation dose responsible for killing 90% of cells) for Photofrin and Photogem irradiated in the three wavelengths. The fact that melanoma cells have problems with the absorption of the light due to poor penetration of the light administrated in PDT (red light: 630 nm) suggests that the melanoma cells can be irradiated with blue and green light producing the same cytotoxic result, only with an adjustment of the light dose. This may offer a means to improve clinical PDT for patients with this diagnosis.