Analysis of the penetration profiles of topically applied substances by laser scanning microscopy

摘要

Tape stripping in combination with spectroscopic measurements is a suitable method for the non-invasive determination of the dermatopharmacokinetics of topically applied substances. The penetration profiles obtained by these methods represent a cut to the stratum corneum, where the distribution of the topically applied substances is shown. For the penetration of these penetration profiles knowledge regarding the penetration pathways is necessary. Laser scanning microscopy is a well-suited method for the analysis of the penetration pathways. In the present paper, the results obtained by the combined tape stripping method and laser scanning microscopy were compared for the interpretation of penetration profiles concerning the penetration pathways of the topically applied substances.