A field-portable on-chip holographic microscope images dense and blended biological samples using multiheight lens-free imaging. Brightfield microscopy is widely used in various fields, including biomedicine. Nevertheless, a fundamental limitation of optical microscopes is that they have a limited field of view (FOV), which makes it labor intensive, tedious, and relatively expensive to detect rare microscopic features of interest (for example, abnormal cells or signatures of parasites). Another limitation is their relatively bulky structure, which makes the technique less suitable for field use.
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