Vitreous humor (VH) is routinely collected at autopsy for the testing of electrolytes and ethanol. In recent years drugs of abuse have been detected in this specimen. In this study the authors assayed 30 VH samples for phencyclidine (PCP) and 50 specimens for cannabinoids. Specimens were screened by immunoassay and then assayed for PCP by GC-FID and cannabinoids by GC/MS. Eighteen (60%) specimens screened positive for PCP using a cutoff of 25 ng/mL. Quantitative analysis showed PCP concentrations in VH that screened positive ranged 30-290 ng/mL. Corresponding blood concentrations were 50-600 ng/mL. VH PCP concentrations in the 12 cases which screened negative were 40-470 ng/mL. False negative results were probably due to matrix effects. All VH specimens screened for cannabinoids were negative. Ten negative screening specimens assayed by GC/MS yielded 1 11-nor-9-carboxy-delta-9-tetrahydrocannabinol positive result at 2 ng/mL. These data indicated that VH maybe a useful specimen for the detection of PCP but not for cannabinoids.
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机译：通常在尸检时收集玻璃体液（VH），以测试电解质和乙醇。近年来，在该标本中发现了滥用药物。在这项研究中，作者分析了30个VH样品中的苯环利定（PCP）和50个样品中的大麻素。通过免疫分析筛选样本，然后通过GC-FID分析PCP和通过GC / MS分析大麻素。使用25 ng / mL的截断值对十八个（60％）标本进行PCP阳性筛查。定量分析显示，VH中PCP浓度筛查的阳性范围为30-290 ng / mL。相应的血液浓度为50-600 ng / mL。筛选出阴性的12例患者的VH PCP浓度为40-470 ng / mL。假阴性结果可能是由于基质效应所致。所有筛查大麻素的VH标本均为阴性。通过GC / MS分析的十个阴性筛查标本在2 ng / mL处产生1 11-nor-9-羧基-delta-9-四氢大麻酚阳性结果。这些数据表明VH可能是检测PCP的有用标本，但不适用于大麻素。
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