Identifying Differences in Solution Conformations of Two Chimeric lgG3 Antibodies through Triple Detection SEC

摘要

Size exclusion chromatography (SEC) coupled to light-scattering, viscometer and refractive index detectors is a common technique used for the characterization of polymers. In this article the solution conformations of two chimeric lgG3 antibodies were studied using this coupled triple detection technique. Conclusions indicate that the results allow hydrodynamic modeling of the antibody structures.