Analysis of Amphetamines and Synthetic Cathinones in Hair Samples Using LC-Tandem Mass Spectrometry and Solid-Phase Extraction

摘要

In this study, the procedure for analyzing amphetamines and synthetic cathinones (also known as bath salts drugs) in hair samples using a mixed-mode solid-phase extraction (SPE) is described. Samples of hair were digested with a dilute solution of base (containing internal standards), neutralized, and diluted with an aqueous phosphate buffer (pH 6). Each sample was applied to a conditioned SPE column, after which the sorbent was rinsed with deionized water, acetic acid, and methanol. After drying, the analytes were eluted and collected from the SPE column with 3 mL of an elution solvent consisting of methylene chloride-isopropanol-ammonium hydroxide. To the eluate, 200 μL of mobile phase was added and the samples were evaporated to the mobile phase for analysis by liquid chromatography-tandem mass spectrometry (LC-MS-MS). Chromatography was performed in gradient mode using a C18 column and a mobile phase consisting of acetonitrile and 0.1% aqueous formic acid. The total run time for each analysis was under 5 min.