DETERMINATION OF SIMULTANEOUS SULFAMETHOXAZOLE AND TRIMETHOPRIM BY ULTRAVIOLET SPECTROPHOTOMETRY WITH MEAN CENTERING OF RATIO SPECTRA

摘要

Objective: Mean centering of ratio spectra (MCR) is analysis method for determination levels of mixture that has maximum wavelength is overlapping, and without preliminary separation. The purpose of this study is a determination of sulfamethoxazole (SLF) and trimethoprim (TRM) mixture by ultraviolet spectrophotometry with MCR. Methods: Absorption spectra of each drug were recorded and divided by the corresponding concentration of divisor, and the ratio spectra were then mean centered. SLF and t TRM concentrations were determined from calibration graphs by measuring at obtained maximum wavelengths 256 nm for SLF and 288 nm for TRM. Result: The result determination of SLF and TRM content in tablet S was 103.31±0.34 and 96.588±2.13, respectively, result of validation method was fulfilled, percentage recovery for SLF was 101.24 and relative standard deviation (RSD) was 0.41, and percentage recovery for TRM was 100.89 and RSD was 0.96. Conclusion: The determination simultaneous by ultraviolet spectrophotometry of MCR was found to be accurate, precise, simple, and rapid method and can be used for SLF and TRM mixture.