RNA editing sites in plant mitochondria can share cis-elements

摘要

RNA editing in flowering plant mitochondria alters numerous C nucleotides in a given mRNA molecule to U residues. To investigate whether neighbouring editing sites can influence each other we analyzed in vitro RNA editing of two sites spaced 30 nt apart. Deletion and competition experiments show that these two sites carry independent essential specificity determinants in the respective upstream 20-30 nucleotides. However, deletion of a an upstream sequence region promoting editing of the upstream site concomitantly decreases RNA editing of the second site 50-70 nucleotides downstream. This result suggests that supporting cis-Itrans-interactions can be effective over larger distances and can affect more than one editing event. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.