Genetic characterization of novel strains of rats derived from crosses between Wistar-Kyoto and spontaneously hypertensive rats, and comparisons with their parental strains.

摘要

Two novel strains of rats have been generated from hybrid crosses of spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats. The 2 new strains were inbred for >25 generations to produce rats that are hypertensive but not hyperactive (WKHT) and hyperactive but not hypertensive (WKHA). The strains were genetically characterized using DNA fingerprinting and simple sequence length polymorphism (SSLP) analyses. The genetic distance between the 2 original strains was greater than that betweenthe novel strains and either of the original strains. Heterozygosities of SSLP marker alleles were extremely rare in WKHA and WKHT, indicating that these strains can be considered as truly inbred (in contrast to WKY rats from 2 sources). It was almost always possible to match the SSLP marker alleles found in WKHA and WKHT rats with similarly sized alleles in the parental SHR and WKY strains, indicating that the WKHA and WKHT strains constitute true mixes of the genomes of SHR and WKY rats. Furthermore,immunogenetic analyses indicated that WKY and WKHT rats belonged to the RT1 l haplotype, whereas SHR and WKHA rats belonged to the k haplotype.