Current methods used for analyzing biomarkers involve expensive and time consuming techniques like the Sandwich ELISA which require lengthy incubation times, high reagent costs, and bulky optical equipment. We have developed a technique involving the use of a micro-channel with integrated electrodes, functionalized with receptors specific to target biomarkers. We have applied our biochip to the rapid electrical detection and quantification of target protein biomarkers using protein functionalized micro-channels. We successfully demonstrate detection of anti-hCG antibody, at a concentration of 1 ng ml~(-1) and a dynamic range of three orders of magnitude, in less than one hour. We envision the use of this technique in a handheld device for multiplex high throughput analysis using an array of micro-channels for probing various protein biomarkers in clinically relevant samples such as human serum for cancer detection.
展开▼
机译：用于分析生物标志物的当前方法涉及昂贵且耗时的技术，例如三明治式ELISA，其需要漫长的孵育时间，高试剂成本和笨重的光学设备。我们已经开发出一种技术，该技术涉及使用带有集成电极的微通道，该电极由针对靶标生物标志物的受体功能化。我们已经将我们的生物芯片应用于使用蛋白质功能化微通道对目标蛋白质生物标志物进行快速电检测和定量分析。我们成功地证明了在不到一小时的时间内检测到浓度为1 ng ml〜（-1）且动态范围为三个数量级的抗hCG抗体。我们设想在手持设备中使用该技术进行多重高通量分析，该分析使用微通道阵列来探测临床相关样品（例如人血清）中的各种蛋白质生物标记物，以检测癌症。
展开▼
