Highly sensitive signal detection of duplex dye-labelled DNA oligonucleotides in a PDMS microfluidic chip:confocal surface-enhanced Raman spectroscopic study

摘要

Rapid and highly sensitive detection of duplex dye-labelled DNA sequences in a PDMS microfluidic channel was investigated using confocal surface enhanced Raman spectroscopy (SERS).This method does not need either an immobilization procedure or a PCR amplification procedure,which are essential for a DNA microarray chip.Furthermore,Raman peaks of each dye-labelled DNA can be easily resolved since they are much narrower than the corresponding broad fluorescence bands.To find the potential applicability of confocal SERS for sensitive bio-detection in a microfluidic channel,the mixture of two different dye-labelled (TAMRA and Cy3) sex determining Y genes,SRY and SPGY1,was adsorbed on silver colloids in the alligator teeth-shaped PDMS microfluidic channel and its SERS signals were measured under flowing conditions.Its major SERS peaks were observable down to the concentration of 10~(-11) M.In the present study,we explore the feasibility of confocal SERS for the highly sensitive detection of duplex dye-labelled DNA oligonucleotides in a PDMS microfluidic chip.